ABSTRACT
Objective To establish the cell model of ethanol-induced liver injury and explore the protective effects of tea poly-phenols (TP)on ethanol-induced liver injury .Methods Cell morphology were observed by microscope ,and then alanine aminotrans-ferase (ALT) ,nmda transaminase (AST) ,gamma GGTP ,GGT and ROS changes were detected .Results Alcohol maked L02 hepa-tocyte fatty degeneration .Compared with ethanol group ,steatosis in TP + ethanol group was lighter ,its ALT ,AST ,GGT content and intracellular ROS reduced .Conclusion TP can decrease cell fatty change degree in vitro experiments ,improue the enzymology indexes ,reduce the generation of reactive oxygen species to avoid liver damage .
ABSTRACT
This study was aimed to detect the effect of genipin and Vitamin E (VitE) on non-alcoholic fatty liver disease. L02 cells were divided into five groups:control group, palmic acid treated group, VitE treated group, genipin treated group, and a combination group. All treatments were terminated at the end of 72 hours. Pathological changes of L02 cells were observed. Mitochondrial membrane potential changes were detected by flow cytometry. MDA, SOD, ALT, AST, GGT, TG in culture medium and expression of UCP2 mRNA and protein in L02 cells were detected. We also studied the effects of genipin and VitE on UCP2 and other related factors such as NF-kappaB and TNF-alpha on the L02 cell model of non-alcoholic fatty liver disease. In combination group, the degree of adipose degeneration of L02 cells mitigated significantly; mitochondrial membrane potential and the level of SOD activity increased; the level of MDA, ALT, AST, GGT, TG and the expression of UCP2, NF-kappaB,TNF-alpha in L02 cells decreased. The use of genipin in combination with VitE can increase mitochondrial membrane potential and markedly relieve the adipose degeneration of liver cells.
Subject(s)
Humans , Cell Line , Drug Synergism , Fatty Liver , Metabolism , Ion Channels , Genetics , Metabolism , Iridoid Glycosides , Pharmacology , Iridoids , Liver , Cell Biology , Membrane Potential, Mitochondrial , Mitochondrial Proteins , Genetics , Metabolism , NF-kappa B , Genetics , Metabolism , Non-alcoholic Fatty Liver Disease , Protective Agents , Pharmacology , RNA, Messenger , Genetics , Metabolism , Tumor Necrosis Factor-alpha , Genetics , Metabolism , Uncoupling Protein 2 , Vitamin E , PharmacologyABSTRACT
Pathology is the bridge of medicine between basic course and clinic course.The building and development of the subject is inseparable from the surgical pathology.Teachers should enrich and enhance professional knowledge,pay attention to the concepts and key points,and handle properly the relationship of the general pathology and the systematic pathology.Multimedia tools for teaching pathology are suitable but still need improvement.